Marker de greutate ADN 1 kb DNA Ladder

Caracteristici standard:
  • Marker de greutate ADN 1 kb DNA Ladder recomanda utilizarea unui gel de 0,8-1,2% realizand separarea optima de 1%.

  • Protocolul este recomandat pentru o banda de gel de 5 mm. Componentele amestecului trebuie scalate in sus si in jos, in functie de latimea benzii.

  • Prezinta o concentratie de 50 ug/ml si poate fi livrat sub forma de 1.25 ml si 3.75 ml.

  • Scara de ADN consta in plasmide brevetate care sunt digerate de enzime de restrictie pana la formarea celor 10 benzi adecvate pentru a fi utilizate ca standarde de greutate moleculara pentru elctroforeza in gel de agaroza.

  • ADN-ul digerat include fragmente cuprinse intre 0,5-10,0 kb. Fragmentul de 3,0 kb a crescut intensitatea pentru a servi ca benzi de referinta.

Descriere

Este disponibil in format Quick-Load Purple-beneficind de benzi mai clare, fara umbre UV si la un pret mai mic.

Prezinta benzi cuprinse intre 500 bp pana la 10 kb, avand un format convenabil, gata de incarcare.

Vine impreuna cu un colorant albastru de bromfenol find usor de identificat benzile de referinta.

1kb ADN lader este un marker de greutate prezent sub forma unui preamestec, pregatit pentru incarcare ce contine ablastru de bromfenol drept colorant de urmarire.


Specificatii complete
Product Name 1 kb DNA Ladder
Concentration:  500 µg/ml
Unit Definition:  X
Shelf Life: 24 months
Storage Temp: 20°C
Storage Conditions: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Specification Version: PS-N3232S/L v1.0
Effective Date: 12-Mar-14
A260/A280 Assay - The ratio of UV absorption of 1 kb DNA Ladder at 260 and 280 nm is between 1.8 and 2.0
DNA Concentration (A260) - The concentration of 1 kb DNA Ladder is between 500 and 550 µg/ml as determined by UV absorption at 260 nm.
Electrophoretic Pattern - The banding pattern of 1 kb DNA Ladder on a 1.2% agarose gel shows discrete, clearly identifiable bands at each size fragment of the marker, when stained with Ethidium Bromide at a concentration of 0.5 µg/ml.
Non-Specific DNase Activity (DNA, 16 hour) - A 50 µl reaction in 1X NEBuffer 2 containing 2.5 µg of 1 kb DNA Ladder incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.