Enzima restrictiva de clasa I Mfe I
Caracteristici standard:
| Product Name | MfeI |
| Concentration: | 10,000 units/ml |
| Unit Definition: | One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl. |
| Shelf Life: | 12 months |
| Storage Temp: | -20 °C |
| Storage Conditions: | 50 mM NaCl, 10 mM Tris-HCl (pH 7.4), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 200 µg/ml BSA |
| Specification Version: | PS-R0589S/L v1.0 |
| Effective Date: | 20-Aug-13 |
| Blue-White Screening (Terminal Integrity) | - A sample of LITMUS28i vector linearized with a 10-fold excess of MfeI, religated and transformed into an E. coli strain expressing the LacZ beta fragment gene results in |
| Endonuclease Activity (Nicking) | - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of supercoiled pUC19 DNA and a minimum of 10 units of MfeI incubated for 4 hours at 37ºC results in |
| Exonuclease Activity (Radioactivity Release) | - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of a mixture of single and doublestranded [ ³H] E. coli DNA and a minimum of 10 units of MfeI incubated for 4 hours at 37ºC releases |
| Ligation and Recutting (Terminal Integrity) | - After a 20-fold over-digestion of Lambda DNA with MfeI, >95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16ºC. Of these ligated fragments, >95% can be recut with MfeI |
| Non-Specific DNase Activity (16 Hour) | - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of Lambda DNA and a minimum of 30 Units of MfeI incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. |
