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Enzima restrictiva de clasa I Fse I - elta90mr

Enzima restrictiva de clasa I Fse I

Caracteristici standard:
  • Se realizeaza digestia in 5-15 minute avand o activitate de 100% in bufer CutSmart in vederea usurari acesteia, venind suplimentar cu un flacon de gel de incarcare violet (6X).

  • Temperatura optima de incubare a enzimei este de 37 oC, iar la o temperatura de 65 enzima se inactiveaza.

  • Concentratia este de 2,000 unitatii/ml, iar dimensiunea este de 100 unitatii.

Descriere
Actioneaza asupra seventei:

                                    5'...GGCCGGCC...3'
                                    3'...CCGGCCGG...5'

Enzima se pastreaza pe gheata, iar atunci cand nu se afla in congelator  trebuie adaugata drept ultima componenta a reactiei. 

In general, se recomanda 5-10 unitati de enzima pe ug ADN si 10-20 unitatii pentru ADN genomic pentru o digestie de o ora.

Specificatii complete