|Product Name||Taq DNA Polymerase with ThermoPol® Buffer|
|Unit Definition:||One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.|
|Shelf Life:||24 months|
|Storage Conditions:||10 mM Tris-HCl , 100 mM KCl , 1 mM DTT , 0.1 mM EDTA , 0.5 % Tween® 20 , 0.5 % IGEPAL® CA-630 , 50 % Glycerol, (pH 7.4 @ 25°C|
|Specification Version:||PS-M0267S/L/X/E v1.0|
|Exonuclease Activity (Radioactivity Release)||- A 50 µl reaction in ThermoPol® Reaction Buffer containing 1 µg of supercoiled PhiX174 DNA and a minimum of 20 units of Taq DNA Polymerase incubated for 4 hours at either 37°C or 75ºC results in|
|Non-Specific DNase Activity (16 Hour)||- A 50 µl reaction in NEBuffer 2 containing 1 µg of T3 DNA in addition to a reaction containing Lambda-HindIII DNA and a minimum of 5 units of Taq DNA Polymerase incubated for A169PCR Amplification (5.0 kb Lambda DNA)16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis|
|PCR Amplification (5.0 kb Lambda DNA)|| - A 50 µl reaction in ThermoPol® Reaction Buffer in the presence of 200 µM dNTPs and
0.2 µM primers containing 5 ng Lambda DNA with 1.25 units of Taq DNA Polymerase for 25 cycles of PCR amplification results in the
expected 5.0 kb product.
|Phosphatase Activity (pNPP)|| - A 200 µl reaction in 1M Diethanolamine, pH 9.8, 0.5 mM MgCl containing 2.5 mM p-Nitrophenyl
Phosphate (pNPP) and a minimum of 100 units Taq DNA Polymerase incubated for 4 hours at 37°C yields <0.0001 unit of alkaline
phosphatase activity as determined by spectrophotometric analysis.
|Protein Purity Assay (SDS-PAGE)|| Taq DNA Polymerase is η 99% pure as determined by SDS-PAGE analysis using Coomassie Blue
|qPCR DNA Contamination (E. coli Genomic)||A minimum of 5 units of Taq DNA Polymerase is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E. coli 16S rRNA locus. Results are quantified using a standard curve generated from purified E. coli genomic DNA. The measured level of E. coli genomic DNA contamination is ζ 1 E. coli genome.|