ADN polimeraza - Taq DNA Polymerase
Taq ADN polimeraza este standardul industrial pentru PCR, fiind ideal pentru aplicatiile de rutina PCR.
Poate fi livrata impreuna cu tamponul ThermoPol ce asigura un randament ridicat al produsului in conditii exigente sau impreuna cu tamponul Taq standard.
Valoare excelenta privind costul per unitate, cu o concentratie de 5,000 de unitati/ml.
Caracteristici standard:
Descriere
Gena pentru Taq ADN polimeraza este de la Thermus aquaticus YT-1 fiind extrasa dintr-o tulpina de E.coli. Tampoanele din componenta
acestei ADN polimeraze au o concentratie de 10X necesitand pastrarea la -20oC.
Taq ADN polimeraza este o ADN polimeraza termostabila, poseda o
activitate de polimerizare 5’-3’ si o activitate de endonucleaza la capul 5’.
Livrarea se face impreuna cu tamponul ce prezinta o concentratie de 10X, care
nu contine detergenti si este proiectat sa fie compatibil cu sistemele de
testare existente.
Tolereaza o gama larga de probe, avand reactii robuste si fiable.
Incorporeaza dUTP, dITP, si nucleotide marcate fluorescent.
Poate fi utilizata pentru urmatoarele aplicatii:
- PCR,
-
extensia
primerilor,
-
DHPLC,
-
analiza microarray, etc.
Specificatii complete
| Product Name | Taq DNA Polymerase with ThermoPol® Buffer |
| Concentration: | 5,000 units/ml |
| Unit Definition: | One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. |
| Shelf Life: | 24 months |
| Storage Temp: | -20°C |
| Storage Conditions: | 10 mM Tris-HCl , 100 mM KCl , 1 mM DTT , 0.1 mM EDTA , 0.5 % Tween® 20 , 0.5 % IGEPAL® CA-630 , 50 % Glycerol, (pH 7.4 @ 25°C |
| Specification Version: | PS-M0267S/L/X/E v1.0 |
| Effective Date: | 02-Dec-15 |
| Exonuclease Activity (Radioactivity Release) | - A 50 µl reaction in ThermoPol® Reaction Buffer containing 1 µg of supercoiled PhiX174 DNA and a minimum of 20 units of Taq DNA Polymerase incubated for 4 hours at either 37°C or 75ºC results in |
| Non-Specific DNase Activity (16 Hour) | - A 50 µl reaction in NEBuffer 2 containing 1 µg of T3 DNA in addition to a reaction containing Lambda-HindIII DNA and a minimum of 5 units of Taq DNA Polymerase incubated for A169PCR Amplification (5.0 kb Lambda DNA)16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis |
| PCR Amplification (5.0 kb Lambda DNA) | - A 50 µl reaction in ThermoPol® Reaction Buffer in the presence of 200 µM dNTPs and 0.2 µM primers containing 5 ng Lambda DNA with 1.25 units of Taq DNA Polymerase for 25 cycles of PCR amplification results in the expected 5.0 kb product. |
| Phosphatase Activity (pNPP) | - A 200 µl reaction in 1M Diethanolamine, pH 9.8, 0.5 mM MgCl containing 2.5 mM p-Nitrophenyl Phosphate (pNPP) and a minimum of 100 units Taq DNA Polymerase incubated for 4 hours at 37°C yields <0.0001 unit of alkaline phosphatase activity as determined by spectrophotometric analysis. |
| Protein Purity Assay (SDS-PAGE) | Taq DNA Polymerase is η 99% pure as determined by SDS-PAGE analysis using Coomassie Blue detection. |
| qPCR DNA Contamination (E. coli Genomic) | A minimum of 5 units of Taq DNA Polymerase is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E. coli 16S rRNA locus. Results are quantified using a standard curve generated from purified E. coli genomic DNA. The measured level of E. coli genomic DNA contamination is ζ 1 E. coli genome. |
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