Enzima restrictiva de clasa III Afl III

Caracteristici standard:
Specificatii complete
Product Name AflIII
Concentration:  10,000 units/ml
Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Shelf Life: 24 months
Storage Temp: -20 °C
Storage Conditions: 500 mM NaCl , 10 mM Tris-HCl (7.4), 1 mM DTT , 0.1 mM EDTA , 50 % Glycerol , 200 µg/ml BSA
Specification Version: PS-R0541S/L v1.0
Effective Date: 01-May-13
Exonuclease Activity (Radioactivity Release) - A 50 µl reaction in NEBuffer 3.1 containing 1 µg of a mixture of single and doublestranded [ ³H] E. coli DNA and a minimum of 50 units of AflIII incubated for 4 hours at 37ºC releases
Ligation and Recutting (Terminal Integrity) - After a 5-fold over-digestion of Lambda DNA with AflIII, >95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16ºC. Of these ligated fragments, >95% can be recut with AflIII.
Non-Specific DNase Activity (16 Hour) - A 50 µl reaction in NEBuffer 3.1 containing 1 µg of Lambda DNA and a minimum of 30 Units of AflIII incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.